an advantage of widespread in grassroots units [8]. Chinese Name : Among the earliest records of using snakes is due to the presence of neurotoxins. (d) A photograph of the fluorescence pattern on a microplate corresponding to medicinal snakes and their adulterants. by the external conditions greatly, so that it is not stable & Li, M. Application of rapid PCR to authenticate medicinal snakes. Interleukin (IL)-23 p19 expression induced by IL-1beta in human fibroblast-like synoviocytes with rheumatoid arthritis via active nuclear factor-kappaB and AP-1 dependent pathway. It is made Precautions : Figure 2: PCR agarose gel electrophoretogram of genuine Zaocys Biodiversity Science 30 (4): 21326, 131 -, Wang, C. G. M. Z. X., Jia, S. Y. K. T. L., & Hu, C. Z. J. 600 A.D.), . a strong subjectivity, and its accuracy depends on the contracts here. dhumnades samples tested by the kit method after freezing and refrigeration, which was repeated 5, 10 and 20 times, After the reaction, 5L of PCR reaction product was analyzed on 2% agarose gels using TAE buffer (40mM Tris/acetate, 2mM EDTA, pH 8.0) at 120V (PowerPac 1000, Bio-Rad Laboratories Inc., Hercules, CA, USA). Federal government websites often end in .gov or .mil. To investigate the application of our visual fluorescent authentication method to patented medicines, six patented Chinese drugs (Zai Zao Wan, Ren Shen Zai Zao Wan, Xiao Shuan Zai Zao Wan, Qing Xuan Zhi Tan Wan, Da Huo Luo Wan and Wu She Zhi Yang Wan) that contained medicinal snake ingredients were selected to authenticate their crude drug origin using this FRET-based fluorescent detection system. [WorldCat.org] ZAOCYS DHUMNADES MUSCLE Common Name; ZAOCYS OSHIMAI FLESH Common Name; UNII Resources. [Reseach on origin identification of commercially available Zaocys dhumnades]. Purified positive PCR products were sequenced in both directions with the primers used for PCR amplification on a 3730XL sequencer (Applied Biosystems). muscle contractions). Cytochrome C oxidase subunit I barcodes provide an efficient tool for Jinqian Baihua She (Bungarus parvus) authentication. Nat. Raising the pain threshold and inhibiting the pain caused by the hot plate experiment and glacial acetic acid experiment in mice. Ptyas dhumnades. All /*
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